Analytical Packing L1 L11 C8 C18 Column HPLC Columns for Chromatography Separation
hplc column selection
1. Column tube specifications: including column length and inner diameter, common specifications include 150mm x 4.6mm. The longer the column length, the better the separation effect, but it will increase the column pressure and analysis time. A shorter column length (such as 50mm) is usually sufficient to provide good separation efficiency.
2. Particle size: Particle size affects separation speed and column efficiency. Smaller particle sizes (such as 1.8 μ m, 3.5 μ m) can provide higher column efficiency, but require higher column pressure. 5 μ m filler is a commonly used choice for analysis.
3. Pore size: Pore size affects the retention characteristics of the sample, and common pore sizes include 60 Å, 120 Å, and 300 Å. Small molecules are suitable for pore sizes of 80-120 Å, while large molecules are suitable for pore sizes of 300 Å.
4. Shape of packing particles: Spherical particles can reduce column pressure and prolong column life in high viscosity mobile phases.
5. Specific surface area: Specific surface area is related to particle size and pore size, which affects the contact area between the sample and the filler, thereby affecting the separation effect.
6. Chemical properties: including silicone matrix, polymer matrix, carbon loading, bonding phase, and end capping. Different chemical properties affect the separation selectivity and stability of chromatographic columns.
7. pH Range: The pH range of a chromatographic column affects its performance under specific conditions.
8. Column efficiency: It is usually measured by the theoretical number of trays and is an important indicator for evaluating the efficiency of chromatographic columns.
9. Separation degree: Separation degree is an indicator of the separation performance of a chromatographic column.
10. Peak symmetry: Peak symmetry is an indicator that describes the degree of symmetry of chromatographic peaks, and a symmetrical peak shape helps improve the accuracy of analysis.
*Tell us your requirements, and we will select the most suitable chromatographic column for you
Product Features
1.Proprietary surface modification for smooth, uniform and inert silica surface.
2.Long column lifetime and more injections.
3.Unique bonding chemistry and end-capping technology.
4.Exceptional lot-to-lot reproducibility.
5.Excellent peak shape, especially for polar, acidic and basic compounds.
6.Available in analytical and prep types, of all dimensions and sample sizes.
7.Series of stationary reversed phase and normal phases for wide selectivity.
8.Best value - high premium products with very competitive price.
Detailed Photos
| HPLC Columns |
Specifications |
| USP Ref. |
Particle size (um) |
Pore Size(A) |
Surface
Area(m2/g) |
C% |
pH Range |
Endcapped |
| C18-Aqueous |
L1 |
3,5,10 |
120 |
320 |
12% |
1.5-10 |
Y |
| C18-Universal |
L1 |
1.8.3,5,10 |
120 |
320 |
17% |
1.5-10 |
Y |
| 300 |
90 |
8% |
| C18-Low pH |
L1 |
5 |
130 |
160 |
10% |
2.0-8.0 |
N |
| C18-Alkaline |
L1 |
3,5,10 |
120 |
320 |
17% |
1.5-10 |
N |
| C8-Universal |
L7 |
1.8,3,5,10 |
120 |
320 |
12% |
1.5-10 |
Y |
| 300 |
90 |
| C8-Low pH |
L7 |
5 |
120 |
320 |
5.50% |
1.0-8.0 |
N |
| C8-Fluorine |
L7 |
5 |
120 |
320 |
12% |
1.5-10 |
Y |
| C1 |
L13 |
5 |
120 |
320 |
4% |
1.5-10 |
Y |
| C4 |
L26 |
3.5 |
120 |
320 |
8% |
1.5-10 |
Y |
| 300 |
90 |
3% |
| SiO2 |
L3 |
5 |
120 |
320 |
N/A |
1.5-10 |
N |
| NH2 |
L8 |
5 |
120 |
320 |
N/A |
1.5-10 |
N |
| CN |
L10 |
5 |
120 |
320 |
6% |
1.5-10 |
N |
| Phenyl |
L11 |
3,5.10 |
120 |
320 |
12% |
1.5-10 |
Y |
| 300 |
90 |
6% |
| Phenyl-Ether |
/ |
5 |
120 |
320 |
12% |
1.5-10 |
Y |
| Polar RP |
L1 |
1.8,3,5,10 |
120 |
320 |
18% |
1.5-10 |
Y |
| 300 |
90 |
| SCX |
/ |
5 |
120 |
320 |
N/A |
2.0-8.0 |
N |
| SAX |
/ |
5 |
120 |
320 |
7.50% |
2.0-8.0 |
N |
| Diol |
/ |
5 |
120 |
320 |
2.50% |
1.5-10 |
N |
| C30 |
L62 |
5 |
120 |
320 |
17% |
1.5-10 |
Y |
| Amino Acid |
/ |
3, 5 |
120 |
320 |
17% |
1.5-10 |
Y |
| PAH |
/ |
5 |
120 |
320 |
22% |
1.5-10 |
Y |
| PFP |
/ |
5 |
120 |
320 |
13% |
1.5-10 |
Y |
| HILIC Amide |
/ |
5 |
120 |
320 |
7% |
2.0-8.0 |
N |
No accident shipping term:
DHL: 5-7 working days;
UPS: 7-9 working days;
FedEx: 7-9 working days;
EMS: 25-30 working days;
China Post: Depends on ship to which country;
Sea: Depends on ship to which country
FAQ
1. Are the columns compatible with my existing chromatography system and detectors?
Our columns are designed with a variety of interface types to ensure compatibility with most chromatography systems and detectors. If you provide us with the specific model number of your existing system, we can recommend the most suitable column model.
2. What is the separation efficiency of the column? How high resolution and selectivity can it achieve?
Our columns use advanced packing technology and unique stationary phase chemistry to ensure high separation efficiency and resolution. We can provide customized columns according to the type of sample you need to analyze and the separation requirements.
3. What are the stationary phase and carrier materials for the columns?
Our columns are made of high quality materials with excellent chemical stability and durability. Whether it is polar or non-polar samples, we have the appropriate column products to meet the analytical needs of different chemical properties.
4. Are the column dimensions (length, inner diameter) and particle size specifications suitable for my analytical needs?
We offer a wide range of column sizes and specifications to suit different analytical methods and sample volumes. You can choose the appropriate column length, inner diameter and particle size according to your analytical needs to achieve the best separation effect.
5. Do you offer cost-effective options, including running cost and lifetime?
Our columns strive to offer competitive pricing while maintaining performance. We focus on long-term customer relationships, so we offer cost-effective products that take into account running costs and column lifetime to ensure that you get cost-effective results over the long term.
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